FastQCFastQC Report
Mon 27 Feb 2023
SRR3128699.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128699.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614648
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187451.1609341478761934No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG66830.4138982614167298TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG66820.41383632841337553TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC59630.36930649900164Illumina Single End Adapter 1 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTCTCTGC26170.16207866977818075No Hit
AGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT20210.12516659977902306No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT18040.11172713805114179No Hit
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT17510.10844468887336435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59850.084.4267961
CGTAGGG2900.081.0366742
GTAGGGA11400.075.444733
TAGGGCG2650.074.487964
ATAGGGC9350.073.387813
CGAAGGG10250.071.992192
AGTAGGG22300.070.185752
AGGGATT14900.070.02445
GTAGAGG10650.069.798751
AGGGAAT19900.068.726245
TATAGGG11900.068.724552
CGGGAAT4250.068.562315
TAGGGCA7550.068.4744344
AGGGCAT13950.068.391885
GTATAGG4550.068.24531
GCAAGGG18750.068.1831742
AGGGTAC6450.067.7650765
TACGAGG3950.066.700811
TAGGGTA5300.066.507114
GGGCGAT8400.066.02156
Produced by FastQC (version 0.11.5)