FastQCFastQC Report
Mon 27 Feb 2023
SRR3128698.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128698.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331272
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211100.9055142428682711No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG41490.1779715108318549No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG38170.1637303583623018No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC37410.16047033550782577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63700.079.491911
ATAACGG2450.071.06991
GAATAGG13300.070.766621
TAGGGCA12150.070.399134
TAGGGAT13100.069.598944
ATAGGGA21000.068.929253
TAGGGTC7250.068.713184
TACGGGT2950.068.5044253
AGTAGGG34800.068.36732
ATAGGGC10800.068.320043
TGTAGGG18050.067.989282
AGAGGGC30150.067.6510543
TATAGGG17100.067.0920262
ATAGAGG15250.066.963781
AGGGCAT20900.066.335795
GATAGGG30850.066.299652
AAGAGGG79600.066.27612
TAGGGTA8000.065.796114
TAGAGGG40000.065.7096252
CCGTACA11200.065.460423
Produced by FastQC (version 0.11.5)