FastQCFastQC Report
Mon 27 Feb 2023
SRR3128697.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128697.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246114
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200740.8937213338236617No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG50730.22585674636282932No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG43770.1948698952947179No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC43010.19148627362636092No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTACAACGT26430.1176698956508886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61500.080.12781
CGTAGGG5850.073.1006852
TAGGGAT11600.072.5155264
TAGGGCA10800.070.054834
ATAGGGA18800.069.490093
ACGGGTA2300.069.468354
TAGGGTA6700.068.7364654
ATAGGGT10150.068.5222553
TATAGGG17700.068.233212
CCGTACA13650.067.821843
GTATAGG8950.067.774091
ATCCGTA14200.067.220961
TCCGTAC14250.066.944842
AAGAGGG70900.066.811352
ATAAGGG29050.066.8097462
AGAGGGC29550.066.792513
GAATAGG14400.066.613881
TAAGGGA17050.066.4245453
ATAGAGG14600.066.345491
AATAGGG32100.066.024862
Produced by FastQC (version 0.11.5)