FastQCFastQC Report
Mon 27 Feb 2023
SRR3128696.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128696.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492175
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245610.9855246922868578No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG56160.22534533088567213No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG50660.20327625467713947No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC50580.20295524993228808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76100.082.357731
AGGGTAC11150.070.391595
ATAGGGA21950.070.229323
TAGGGCA11750.069.5974
GAATAGG13500.069.0106661
ATAGCGG5650.068.288911
GTATAGG9450.068.2140051
CGTAGGG5750.067.8541642
TAGACGG4050.067.384151
AGAGGGC34350.067.315873
ATAAGGG32500.066.67822
TATAGGG18700.066.3633962
TGTAGGG19250.066.176662
AAGAGGG87650.066.126792
TAGAGGG43200.066.049762
AGTAGGG36000.065.940942
CGAAGGG26450.065.934782
ATAGGGC11150.065.755013
AATAGGG32050.065.7076952
TAGCGGG13550.065.567532
Produced by FastQC (version 0.11.5)