FastQCFastQC Report
Mon 27 Feb 2023
SRR3128695.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128695.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784768
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239960.8616875804375805No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG45300.16267064258135688TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG43520.15627872770729914TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC42880.15398051112336827TruSeq Adapter, Index 27 (100% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42660.15319049917264205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG6700.082.105542
CGTTTTT76900.081.6868061
AGGGATG50750.074.361125
ATAGGGC16000.074.313373
AGTAGGG45650.071.067282
GGTAAGG17050.070.650061
TAGGGCA15450.070.5709464
TAGAGGG45050.069.6133352
GAGGGAT40400.069.331614
TAGCGGG14650.069.323032
AGGGAAT34900.068.94635
TAGGGTA9050.068.547524
TAGGGCG5350.068.51844
GTAGGGA22300.068.49283
TATAGGG22400.068.007752
TAAGGGA26700.067.7666243
ATAGCGG5350.067.722651
TAGGGAT23450.067.338414
ATAGGGA28100.067.066323
GTAGGGC13050.066.983663
Produced by FastQC (version 0.11.5)