FastQCFastQC Report
Mon 27 Feb 2023
SRR3128694.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128694.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607612
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220150.8442590385379419No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG50660.19427736948595115TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG49380.1893686637429188TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC47610.18258084408263192TruSeq Adapter, Index 27 (100% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41640.15968633370302024No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTC30170.11569972833381653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG7150.084.1340262
CGTTTTT74750.081.725441
AGGGATG43350.073.5035555
ATAGGGC11950.072.363183
AGGGAAT30700.071.490165
GTACGGG5350.071.1539462
AAGGGCA41950.069.796264
TAGGGCA13150.068.977524
GAATAGG13300.068.951271
AGTACGG2600.068.733671
TAAGGGA22500.068.719673
ATAGGGA25100.068.529113
TAGAGGG47500.068.071072
TGAAGGG38650.067.850412
GGTAAGG14350.067.838731
AGTAGGG39650.067.442992
AGGGCAT24650.067.110965
ATAGCGG6250.066.968311
AGGGTAC11800.066.9106145
AAGGGTA17750.066.723414
Produced by FastQC (version 0.11.5)