FastQCFastQC Report
Mon 27 Feb 2023
SRR3128693.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128693.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2917409
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273410.9371671918472864No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG56640.19414487307059106TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG55690.19088855899190002TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC54440.1866039352041486TruSeq Adapter, Index 27 (100% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47140.1615817322836805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86600.083.24021
CGTAGGG6900.076.988682
GTACGGG6250.074.465082
TAGGGTA7550.072.832424
GGTAAGG15750.072.5968861
AGGGAAT34400.072.54745
ATAGGGC14600.072.4295043
AGGGATG50800.072.348395
TAGGGCA16800.072.176574
TAAGGGA24950.072.14633
ATAGGGA28850.071.19043
GTAGGGT10650.069.725773
AGGGTAC12250.069.442975
AGTACGG3000.069.0118561
AAGGGTA19900.068.726744
AAGAGGG94900.067.766862
TAGAGGG48500.067.656752
TAGACGG4200.067.219331
AGGGTAT15400.067.1415
GAGGGAT42550.067.0463264
Produced by FastQC (version 0.11.5)