FastQCFastQC Report
Mon 27 Feb 2023
SRR3128692.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128692.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657879
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283911.0681825621106154No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG30890.11622049009755522TruSeq Adapter, Index 23 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30620.11520464249877439No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC28470.10711548569366779TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG27990.1053095344069463TruSeq Adapter, Index 23 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97300.083.902851
ATAGGGC16700.076.263453
ATAGCGG5850.074.845821
TAGGGCA14350.072.377574
CGTAGGG8800.070.530262
AGTAGGG40150.070.500992
AGGGATG40450.070.40735
GTAGGGC11350.068.7346653
GAGGGAT33750.067.813914
GTAGGGA22950.067.37163
AGGGCAT23600.066.909975
AGGGAAT29600.065.8900765
ATAAGGG30700.065.858882
TAAGGGA22000.065.5811843
AAGAGGG87650.065.393722
ACGGGTA3100.065.188414
ACTAGGG13200.064.830862
TCCGTAC11150.064.520962
AAGGGCG10450.064.3107154
TATAGGG20400.064.307012
Produced by FastQC (version 0.11.5)