FastQCFastQC Report
Mon 27 Feb 2023
SRR3128691.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128691.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489671
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258791.0394546106694418No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG34700.13937584524220267TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC32620.13102132771759803TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG30930.12423328222885674TruSeq Adapter, Index 23 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28550.11467378621512642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90750.083.7276461
ATAGGGC14000.078.2107543
CGTAGGG7050.074.656472
TAGGGCA12050.072.92784
AGTAGGG40850.071.8996052
AGGGATG37300.069.801645
AGGGCAT22350.068.970135
ATAAGGG29700.067.879622
CCGTACA12050.066.687993
ATAGCGG5150.066.648411
TAGGGTA6700.066.632694
TAAGGGA18550.066.627023
AAGAGGG81500.066.2523042
AGAGGGC36750.065.727083
TAGGGAT17900.065.633494
GTAGGGC11750.065.591043
AGGGAAT25150.065.21575
GAATAGG13450.065.022671
GTAGGGT8600.064.479573
ATGCGGG12800.064.249042
Produced by FastQC (version 0.11.5)