FastQCFastQC Report
Mon 27 Feb 2023
SRR3128690.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128690.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804403
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323631.154006752952411No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG39660.1414204734483596TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC35530.1266936314074689TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG33460.11931238128043652TruSeq Adapter, Index 23 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32760.11681630635825165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108850.084.462251
ATAGCGG5100.077.4953461
ATAGGGC18550.075.247313
CGTAGGG8950.072.480242
TAGGGCA15250.072.422994
AGGGATG39900.071.4980555
TAGGGTA8800.069.9627764
AGTAGGG45100.068.1655352
GAATAGG16600.067.45831
GTAGGGC13100.066.7298363
AGGGCAT24800.066.517195
TAGCGGG15400.065.932052
TAAGAGG19400.065.725531
ATAAGGG36200.065.706082
ACGGGTA3300.065.5122154
GTAAAGG21050.065.4910661
AAGGGTA19100.065.452574
TATAGGG22250.065.281792
AAGAGGG95950.065.158382
GTAGGGT10900.065.107183
Produced by FastQC (version 0.11.5)