FastQCFastQC Report
Mon 27 Feb 2023
SRR3128689.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128689.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678056
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGT87661.2928135729202308No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77471.1425310003893483No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG54240.7999339287610463No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC43810.6461118255719291No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35510.5237030569746451No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG33690.49686161614969854No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA31000.45718937668865106No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATG30710.45291244380995077No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTTAGCGGTTC19560.2884717486461295No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT17340.25573109005745837No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG13560.19998348219026157No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG12570.18538291822504338No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG12410.18302323111955354No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT11230.16562053871656618No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGT10630.1567717120709794No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG10620.15662423162688627No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGC10390.15323218141274467No Hit
AGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT9840.14512075698762344No Hit
GGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT8620.12712814280826362No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTTA7760.11444482461625588No Hit
TAGACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT7410.10928300907299693No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT7390.10898804818481068No Hit
GGAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT7290.10751324374387956No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT7290.10751324374387956No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCG7090.10456363486201731No Hit
GAACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT7070.10426867397383106No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG6980.10294134997699306No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC6800.10028670198331702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAGG600.094.1219941
GCGATTG254.9220944E-793.990128
CGTTTTT40450.086.6761
TAGGGTA1150.085.817064
TAGACGG1400.084.037491
TAGGGCA2800.083.919754
GGTCACT900.083.5467767
AGGGATG12200.082.819165
CGAGGGA4600.081.730543
AGGGAAT14950.081.730545
CGCTACG6850.081.641053
CGAAGGG3150.080.604562
GTAGGGC3100.080.346393
CGTAGGG1000.079.932862
TAAGGGA6450.078.68943
AGGGCAA6050.078.454565
TACGACC7150.077.558286
GTAGGGT2000.077.541853
AGTAGGG9200.077.173032
GCTACGA7200.077.0196844
Produced by FastQC (version 0.11.5)