FastQCFastQC Report
Mon 27 Feb 2023
SRR3128688.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128688.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680938
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGT116041.704119905189606No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69551.021385206876397No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG62930.9241663705065659No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC49000.719595616634701No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATG41610.6110688491463246No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG39350.5778793370321527No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA36860.5413121312072465No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33620.49373070676038056No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTTAGCGGTTC25780.3785954080988284No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT19240.2825514217153397No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG17660.25934813448507793No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGT14730.21631925373528865No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGC14100.20706731009284252No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT12840.18856342280795022No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG12780.18768228531819345No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG12530.18401087911087355No Hit
AGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT10910.16022016688744056No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG10140.14891223576889526No Hit
GGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT10050.14759052953426008No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCG9630.14142256710596265No Hit
GAACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT8880.13040834848400293No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT8560.12570894853863346No Hit
TAGACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT8470.12438724230399831No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT8220.12071583609667841No Hit
GGAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT8110.11910041736545765No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTTA7900.11601643615130892No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT7850.11528215490984495No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCG7800.11454787366838098No Hit
ATCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC7600.11161074870252505No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT7100.10426793628788524No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG7000.1027993738049573No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT6900.10133081132202933No Hit
GACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC6880.10103709882544373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG850.088.513321
CGTTTTT41550.087.0287861
GCGGGAT1700.085.70324
CGCTACG5850.083.552953
GCTACGA5750.083.3713154
CGGGATG2050.082.5340045
AGGGAAT15500.082.171635
TCCGCTA6050.080.832421
TCCGTAC46450.079.528192
CTACGAC6050.079.23725
CCGTACA44100.079.1834643
CGTACAC43650.079.030754
GGCGATG1250.078.951737
ATCCGTA47300.078.8351061
AGGGATG10750.078.695225
AGCACGG600.078.371171
CGTAGGG900.078.330892
CGGGATC1500.078.330895
GGGAATG20450.077.903836
GGAATGC7200.077.6724247
Produced by FastQC (version 0.11.5)