FastQCFastQC Report
Mon 27 Feb 2023
SRR3128687.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128687.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726492
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89961.2382792928208433No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG69340.9544496016473685No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGT60540.8333195685568457No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC52930.7285696194865188No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG43200.5946383442625659No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38680.5324215545387974No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA23100.31796633686262205No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT21070.29002384059287645No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATG20390.28066379258133606No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT14110.19422099623946307No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG13880.1910550976473244No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG13190.1815574018709084No Hit
AGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT13070.17990562869240131No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTTAGCGGTTC12990.17880444657339653No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG11600.15967140725568899No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG11080.1525137234821581No Hit
GGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT9970.13723482158096717No Hit
TAGACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT9710.13365597969420173No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT9420.1296641945128095No Hit
GAACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT9390.1292512512181827No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT9280.12773712580455118No Hit
GGAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT9160.12608535262604406No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCG8690.11961590767689113No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTTA8530.11741354343888163No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT8240.11342175825748942No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG8110.11163233731410668No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT7790.10722760883808768No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT7780.10708996107321209No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC7720.10626407448395853No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGC7620.10488759683520259No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGT7390.10172169824306393No Hit
GACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC7370.10144640271331275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTA1200.090.0793154
CGTTTTT47350.088.230761
AGGGATG11000.085.450745
CGTAGGG950.084.118872
AGGGAAT17050.083.7968445
CGAAGGG2900.082.668552
AGGGCAT5950.082.147595
CGCTACG7900.082.09763
TCCGCTA8050.080.651091
GCTACGA8050.080.567834
TACGACC8100.080.0704966
GAAGGGC7200.079.635343
AAGGGCA8750.078.9564744
CTACGAC8250.078.614675
AGTAAGG3250.078.1695251
AGGGCGC1150.077.6487055
AGGGTAG1700.077.408315
GTAGGGC2800.077.210833
CGAGGGA4450.077.097683
GATAAGG3950.076.2271961
Produced by FastQC (version 0.11.5)