FastQCFastQC Report
Mon 27 Feb 2023
SRR3128686.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128686.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520713
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240040.9522702505203885No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG67730.26869381797927805TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG64530.25599899710915125TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC59760.23707577974961846TruSeq Adapter, Index 21 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGATAAGTG39160.15535287039817702No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA28350.11246817864627985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73350.085.264621
CGTAGGG6650.080.600772
TAGGGCA14700.077.6882554
ATAGGGC16600.074.4584663
GTAGGGC11400.073.79293
AGTAGGG38650.072.502462
TAGACGG2150.072.230311
TAGGGCG4650.071.758254
AGGGATG35950.071.638765
AGGGAAT36650.071.296345
GTAGGGA22200.070.918293
ATAGGGA24000.069.7116243
AAGGGTA19350.069.462684
TATAGGG21250.069.032192
TAGCGGG12100.068.77712
AGGGCAT21900.068.456235
TAGAGGG44050.068.417652
TAGGGAT18800.067.995084
CGAAGGG22050.067.5937652
CCGTACA20850.066.944793
Produced by FastQC (version 0.11.5)