FastQCFastQC Report
Mon 27 Feb 2023
SRR3128685.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128685.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386779
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219290.9187696053970644No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG78000.32680026093743914TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG72470.3036309603863617TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC66890.2802521724885295TruSeq Adapter, Index 21 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGATAAGTG48670.20391498333109181No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA35060.1468925275444438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68450.084.021961
CGTAGGG5550.081.293182
ATAGGGC13950.074.455023
AGGGAAT34650.074.325025
TAGGGCA12550.073.3986744
CCGTACA25700.071.319353
GTAGGGC8800.071.0304953
ATAGGGA19800.070.73383
AGGGATG32750.070.6011055
CGAAGGG20000.070.496432
AGTAGGG35100.070.429482
ATAGCGG3950.070.241651
TAGGGTA7500.069.556484
AGGGTAC10800.069.188055
AGGGCAT20400.068.880885
TCCGTAC27400.068.781192
AAGGGTA18400.068.708484
TAAGGGA20550.068.6096653
GTAGGGA19650.068.164493
AGTAAGG14400.067.600161
Produced by FastQC (version 0.11.5)