FastQCFastQC Report
Mon 27 Feb 2023
SRR3128684.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128684.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668095
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278001.0419419098645288No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG86110.322739632584297TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG84410.31636804536570096TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC80340.30111371596588576TruSeq Adapter, Index 21 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT27880.10449403038497505No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGATAAGTG27230.10205783527198245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78850.085.2695851
TAGACGG3350.078.651471
CGTAGGG7650.078.030812
ACGGGTA2650.076.260144
TAGGGCA16600.075.309294
ATAGGGA25000.075.1960453
GTAGGGC11100.074.9423
AGGGAAT39200.073.25385
AGGGATG36000.072.454515
AGTAGGG42550.071.802182
ATAGGGC17500.071.167683
CGAAGGG26150.070.818762
GTAGGGA25350.070.449943
TATAGGG21550.069.359142
TAGCGGG12400.068.987992
TAGGGAT19100.068.1587144
TAGAGGG45650.067.955872
GAGGGTA14650.067.689274
GGAATGC20450.067.336317
AGAGGGC36400.067.139333
Produced by FastQC (version 0.11.5)