FastQCFastQC Report
Mon 27 Feb 2023
SRR3128683.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128683.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3157477
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272630.8634425523923057No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC101150.32035071039313984No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC45160.14302558656800984No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG40470.12817195501344902TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG38320.12136272093193395TruSeq Adapter, Index 14 (95% over 21bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC34520.10932779557855846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96550.082.8710251
AGGGATG73100.078.626375
TAGGGCA21300.075.016794
GGATGGC52100.072.0722667
CGTAGGG9600.071.997672
ATAGGGC23050.071.7680053
ATAGGGA38400.070.983253
CGCTACG21000.070.717513
GAGGGAT55900.070.619824
TAAGGGA33950.070.182263
TAGCGGG15650.070.002572
GCTACGA21350.069.998444
TAGACGG4850.069.870981
CTACGAC21400.069.834895
AGTACGG4050.069.727211
AAGGGAT71350.069.5551154
TCCGCTA21550.069.452191
AGTAGGG49500.069.340992
AGGGAAT45200.069.1420755
ACGACCA21450.069.0148247
Produced by FastQC (version 0.11.5)