FastQCFastQC Report
Mon 27 Feb 2023
SRR3128681.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128681.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470559
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316650.9123890416500626No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC118840.34242322346342474No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC52540.15138771592703076No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG49100.14147576802468997TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG47470.13677911829189476TruSeq Adapter, Index 14 (95% over 21bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC36570.10537207406645442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105650.085.2494661
CGTAGGG10200.076.960162
AGGGATG76300.076.44135
CTACGAC23900.073.151945
TCCGCTA24300.072.617991
AGTACGG4950.072.24831
TAAGGGA38500.072.1452263
ACGACCA23950.071.625597
AAGGGAT74750.071.48754
TAGGGCA20950.071.338494
TAGCGGG18650.070.823462
GGATGGC58300.070.779387
GAGGGAT61000.070.728374
GGTAAGG21950.069.8879551
CGAGGGA22450.069.712183
AGGGCAT35850.069.481235
AGGGAAT53700.069.315725
AGTAGGG55800.068.9920962
AGACGGG23900.068.8366552
TAGGGCG5600.068.818734
Produced by FastQC (version 0.11.5)