FastQCFastQC Report
Mon 27 Feb 2023
SRR3128680.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128680.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2690103
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249930.9290722325502034No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG53720.19969495591804476No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG49860.18534606295744066No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC46400.17248410190985253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77100.082.339121
ATAGCGG5600.074.7912141
AGGGCAT22150.074.684055
GTACGGG6500.073.064972
TAGGGCA13350.072.869884
CGTAGGG6900.072.236712
AGGGAAT30250.072.0861055
GAATAGG15100.069.187021
CGGGTAT3150.068.628775
AGGGATG38100.068.211745
ATAGGGC15050.067.4491043
AGAGGGC36000.067.099543
TAAGGGA24600.067.054973
AGTAGGG40150.066.521792
GTAGGGA20150.066.470483
AAGGGAT39050.066.1912464
AATAGGG33150.065.674782
AAGAGGG88900.065.270152
AGAAGGG85100.065.2561652
ATGCGGG13800.065.0811842
Produced by FastQC (version 0.11.5)