FastQCFastQC Report
Mon 27 Feb 2023
SRR3128679.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128679.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2536232
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230110.9072908156667056No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG61700.24327427459317602No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG56020.2208788470455384No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC53460.21078513322125106No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCTTAACA31600.12459428001854721No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68950.082.10311
ATAGGGC14650.072.817023
ATAGCGG5050.072.622391
TAGGGCG3950.071.3864754
CGAAGGG22700.070.80192
ACGGGTA3550.070.1631854
AGGGAAT29100.069.76745
AGGGATG33900.069.7316745
GTAGGGA18950.069.437413
AGGGCAT20850.069.423495
GTACGGG5900.069.29662
CGTAGGG6300.068.626512
TAGGGCA12400.068.5991444
GAATAGG14450.068.0057751
AAGGGTA16100.066.845384
AATAGGG35450.066.680122
GGTAAGG12900.066.335961
TAGCGGG12800.066.0856552
AAGGGAT36150.066.041544
TAAGGGA21350.065.5938343
Produced by FastQC (version 0.11.5)