FastQCFastQC Report
Mon 27 Feb 2023
SRR3128678.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128678.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843596
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292031.026974295926707No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG69040.24279117005369258No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG64550.22700130398270357No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC61270.21546661340077844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83550.082.782441
ATAGCGG4750.077.262431
CGTAGGG6600.075.497392
AGGGCAT23850.073.109115
AGGGAAT34200.072.6968465
ATAGGGC17350.071.784783
CGGGTAT3700.069.862955
ACGGGAT8050.069.4763644
TAGGGCA15500.069.13364
GTAGGGA20200.069.102073
AGGGATG41750.069.00655
GAATAGG16700.068.7449341
TAGCGGG14650.068.6666952
CGAAGGG28300.067.770982
AGTAGGG42150.067.026552
ACGGGTA4250.066.351134
TAAGGGA26250.066.245813
AAGGGCG10700.065.8860554
AAGGGCA39350.065.690714
TAGACGG3450.065.4620361
Produced by FastQC (version 0.11.5)