FastQCFastQC Report
Mon 27 Feb 2023
SRR3128677.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128677.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159356
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123721.067144173144401No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG62140.5359872204913763No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGGAATAT61510.5305531691732307No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC59720.515113563047071No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC51780.446627265481871No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG49720.4288587802193632No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA27190.23452675450853752No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATG23540.20304375877642414No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT17950.1548273351757355No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT16870.14551181863034307No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG16740.14439050645358287No Hit
TAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATA15540.13403993251425791No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT15430.13309112990315314No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGGGAATATTC13980.1205841863931355No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGGG13340.1150638802921622No Hit
AGAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT12650.10911230027705038No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT12340.10643840200939142No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC12170.10497207070132039No Hit
GGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT11690.10083184112559042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG11650.087.9403463
CGTTTTT52950.087.38161
AGTACGG1350.087.164311
ACGGGTA1600.085.17964
ATAGCGG1950.084.482321
CGTAGGG3400.084.370292
AGGGATG29200.083.047095
CTACGAC12600.080.936945
GGATGGC23400.080.73617
CGACCAA12400.080.726398
AGGGAAT26050.080.460875
TACGGGT1350.080.066653
GCTACGA12800.079.67234
ACGACCA12600.079.4450157
TCCGCTA13200.078.4478761
CGGGTAG1200.078.326075
GCGACTT301.4569523E-678.326078
GGGTATC5850.077.92446
GGGAATG34250.077.800086
TACGAGG1400.077.327191
Produced by FastQC (version 0.11.5)