FastQCFastQC Report
Mon 27 Feb 2023
SRR3128676.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128676.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112745
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112171.0080476659072835No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGGAATAT81090.728738390197215No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG68430.6149656929485192No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC60650.5450485061716746No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG57270.514673173098958No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC55120.49535158549353175No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA31120.2796687471073786No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATG29050.2610661022965729No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG20020.17991543435378277No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT19410.17443349554480136No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT18240.1639189571734764No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT17940.1612229216936495No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGGGAATATTC17760.15960530040575335No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGGG13900.1249163105653137No Hit
AGAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT13740.1234784249760727No Hit
TAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATA13590.12213040723615923No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT13320.119703975304315No Hit
GGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT13040.11718767552314321No Hit
TAGACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT11640.10460617661728429No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGGGAATATTCGT11340.10191014113745736No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC11140.10011278415090609No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTA201.8387387E-593.991068
CGTTTTT45300.087.410441
ATAGCGG2000.084.649021
AGGGATG24400.083.986945
CTAACGG452.7284841E-1183.6039661
CGCTACG10850.082.736893
CTACGAC11000.081.612335
GCTACGA11000.081.608664
AGGGTAT7000.081.2460865
GGATGGC22200.081.077877
TACGGGT1050.080.5713
ACGACCA10850.080.130637
AGGGAAT22650.080.10035
CGACCAA10950.079.8280268
AGTACGG1600.079.358451
AAGGGAT22550.079.2013554
GGAATGC14600.079.184257
TCCGCTA11700.078.378711
TAAGGGA12000.078.332923
CGTAGGG2950.078.067382
Produced by FastQC (version 0.11.5)