FastQCFastQC Report
Mon 27 Feb 2023
SRR3128675.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128675.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219065
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145971.1973930840439189No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG77890.6389322964731167No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC69660.5714215402788202No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC63400.5200707099293311No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG61490.5044029645671068No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGGAATAT42290.34690521014055853No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT21380.17538031196039588No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT20520.16832572504337343No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT19600.16077895764376798No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG18720.1535603105658845No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA18330.15036113742909527No Hit
TAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATA15800.1296075270801803No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGGG15490.127064594586835No Hit
AGAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT14830.12165060927842239No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT14790.12132248895670042No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATG14320.1174670751764672No Hit
GGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT13460.11041248825944473No Hit
TAGACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT13050.10704925496179449No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC12820.10516256311189312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG2150.089.713671
CGTTTTT57000.087.652221
CGTAGGG2850.087.411782
TACGGGT1350.087.034563
CGCTACG12600.086.537233
AGGGAAT28250.083.8489465
AGGGATG28650.082.5142445
GGATGGC23350.082.323157
CTACGAC13300.081.9826355
TAGGGCG1500.081.4643554
TCCGCTA13700.081.384371
GCTACGA13450.081.068344
TAAGGGA13700.080.9612053
GCGGGAT7500.080.837714
TAGGGCA5700.080.8047264
CATACGG353.839341E-880.648541
AGTACGG1350.080.15071
TACGACC13600.079.828626
GTAGGGA12200.079.3584063
CGACCAA13450.079.3244258
Produced by FastQC (version 0.11.5)