FastQCFastQC Report
Mon 27 Feb 2023
SRR3128674.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128674.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910122
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83820.920975429667671No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC60070.6600214037238964No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCC49990.5492670213443912No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG46840.5146562768507958No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC37790.4152190585437996No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG32970.3622591256996315No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA19810.21766312648194416No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG17860.19623742750971848No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT14800.16261556143022585No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTT13900.1527267772891986No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGTTGTCCCTC11770.12932332148876743No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG11700.1285541938333542No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT11530.1266863123844935No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT9920.1089963763099892No Hit
TAGACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCT9810.107787747137197No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT9300.10218410279061489No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG1500.087.728264
CGTTTTT47300.086.984011
CGCTACG11400.086.161683
ACGGGTA1100.085.449614
GCTACGA11650.084.7161254
TCCGCTA11900.084.260061
TACGACC11700.083.550726
CGACCAA11550.083.4150858
CTACGAC11900.082.936385
ACGTAGG1150.081.869341
TACGAGG1450.081.163561
ACGACCA11950.080.622957
GGTACGA353.8653525E-880.566777
CGTAGGG1550.078.8861542
CGAAGGG7750.078.8861542
ATAGGGC6650.078.446593
TAAGGGC6200.077.318113
AGGGATT11250.077.284425
AAGGGAC7500.077.075544
ATAGCGG551.8189894E-1277.03161
Produced by FastQC (version 0.11.5)