FastQCFastQC Report
Mon 27 Feb 2023
SRR3128673.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128673.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888790
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78620.8845734087917281No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCC66250.7453954252410581No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC58310.6560604867291486No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG53510.6020544785607399No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC44100.49618020004725527No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG38230.43013535255797203No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24730.27824345458432254No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG23840.2682298405697634No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT16000.18002002722802912No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGTTGTCCCTC15760.1773197268196087No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTT14640.16471832491364663No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG13980.15729249879049045No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT12380.1392904960676875No Hit
TAGACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCT10670.12005085565769191No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT10460.11768809280032404No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT9740.10958719157506272No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT9510.10699940368365982No Hit
GAACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT9310.10474915334330943No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT9190.10339900313909922No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGT9050.10182382790085397No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAA156.902514E-493.997198
CGTTTTT42550.087.298721
CGGGTAT1000.084.5974665
TAGGGCG1300.083.146684
TACGACC10400.082.2475366
ACGGGTA1200.082.242914
CGCTACG10550.081.5190353
GCTACGA10550.081.5190354
CGGGAAT3100.080.352435
CGTAGGG2050.080.2369842
TCGAAGG1700.080.2101751
GGGAATG22450.079.970886
TCCGCTA10900.079.804171
ACGACCA10500.079.2262047
CTACGAC11000.078.188575
AGGGAAT19850.077.186615
AATGCGG1100.076.941421
CGACCAA10800.076.155138
GGGCGAT3150.076.092966
AGAGGGA22650.075.9404753
Produced by FastQC (version 0.11.5)