FastQCFastQC Report
Mon 27 Feb 2023
SRR3128672.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128672.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984056
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100471.020978480899461No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC67050.6813636622306047No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG57470.5840114790215191No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC46400.47151788109619774No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG42480.43168274976220866No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCC36380.36969440763533784No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT17550.1783435089059972No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA15160.15405627322022322No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTT15000.1524303494923053No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT14260.14491045225068494No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG12510.12712691147658264No Hit
TAGACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCT12170.12367182355475706No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG11550.11737136910907509No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT11440.11625354654613153No Hit
GAACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT10660.10832716837253165No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT10020.10182347346085995No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC156.903236E-493.995487
CGTTTTT49850.087.489351
CTACGAC12300.085.971475
TCCGCTA12600.085.134371
GCTACGA12400.084.899144
CGCTACG12450.084.558183
CGTAGGG2350.084.01732
TAGGGCG1250.082.716024
ACGGGTA1600.082.246044
TACGACC12950.081.65636
CGACCAA12850.081.194548
ACGACCA12850.080.82887
AGGGATG18250.080.604345
GGGCGAT3650.079.831786
AAGGGAC8750.079.4933244
AGTTAGG4100.079.1782461
CGGGTAA950.079.154095
GTAGGGA8550.079.154083
TAAGGGA8850.079.126143
TACGAGG2100.078.413231
Produced by FastQC (version 0.11.5)