FastQCFastQC Report
Mon 27 Feb 2023
SRR3128671.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128671.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622498
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268131.022422133401055No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43770.16690193853341356No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC31640.1206483284257986Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG31370.11961877568638757No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG27600.105243168917574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92850.082.957961
GAATAGG16400.075.170731
ATAACGG2950.071.776421
AGGGATG42150.071.1324165
CGTAGGG6350.071.0761952
ATAGGGC15300.070.952063
TAGGGCA12950.070.400614
GGTAAGG13700.070.0649641
ACGGGAT8050.070.0535054
CGAAGGG28500.068.128982
ATAGGGA23900.068.033483
TAGGGTA7600.068.017954
CCGTACA12200.067.024543
ACGGGTA3300.066.931164
TAGGGTC5700.066.7812654
TAAGGGA23750.066.484463
TAGCGGG13800.066.0920642
AGTAGGG39650.066.044812
AAGAGGG88150.065.974192
AAGGGTA18250.065.920664
Produced by FastQC (version 0.11.5)