FastQCFastQC Report
Mon 27 Feb 2023
SRR3128670.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128670.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488560
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246790.991698010094191No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41800.1679686244253705No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC37030.14880091297778636Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG34920.1403221139936349No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG32490.13055743080335616No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAGCAAGA27830.11183174205162826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80500.082.658821
GAATAGG16050.072.661651
ATAGGGC14200.072.476363
ATAGGGA23100.072.219913
AGGGATG40700.071.8185355
ACGGGTA3000.070.49074
AGGGTAC9300.069.732745
CCGTACA16050.069.685513
CGAAGGG27000.069.6204452
AGGGAAT24050.069.5625465
TAAGGGA23450.068.7372063
CGTACAC16350.068.6943054
AAGGGTA16300.068.3284154
AGGGACT29100.068.149095
AGTAGGG37900.068.0728152
TAGAGGG44350.067.815182
ATAGAGG16600.067.4213641
TCCGTAC17050.067.252122
TAGGGCA11950.067.246364
GGTAAGG14200.066.563531
Produced by FastQC (version 0.11.5)