FastQCFastQC Report
Mon 27 Feb 2023
SRR3128669.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128669.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800731
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309851.1063183147542552No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47670.17020556419020608No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG41980.1498894395784529No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC40570.144855039630725Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG37260.13303669649102323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101900.084.595771
CGTAGGG7050.078.008892
GAATAGG17050.073.9618451
AGGGTAC11850.073.76875
AGGGAAT31500.071.76495
ACGGGAT8600.071.5896764
ATAGGGA25350.071.562743
AGGGATG46350.071.485345
AGTAGGG40050.070.537382
GGTAAGG14250.070.333481
TAGGGCA13700.069.2960364
GTAGGGT9500.069.259923
TAAGGGA25700.069.125173
ATAAGGG36700.069.035112
AGAGGGC37600.067.9968263
ACGGGTA3050.067.800114
GTAGGGA20350.067.66763
AAGGGTA20150.067.639524
AAGGGAT49350.067.425424
ATAGGGC16400.067.344413
Produced by FastQC (version 0.11.5)