FastQCFastQC Report
Mon 27 Feb 2023
SRR3128668.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128668.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2752348
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255640.9288069677235581No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG57080.20738656594296942RNA PCR Primer, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG52770.1917272089139891RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC45680.16596738493824184RNA PCR Primer, Index 38 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28850.10481959403389397No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27640.1004233476290062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84300.081.692171
TAGACGG3600.078.4535141
TATAGGG20500.072.2593152
ATAGGGC15650.071.771563
CGTAGGG6150.071.1123352
AGTAGGG37950.070.012222
TAGGGCA14850.069.941454
CGGGTAT3100.069.7372745
TAGGGTA9650.069.643014
AGGGCAT24100.069.032725
GTAGGGA20850.068.74843
GTAGGGC10600.068.721823
TAGCGGG14450.068.667582
AGAGGGC40150.068.593173
AGGGATG30850.068.095935
ATAGGGT11500.067.021613
AGACGGG19750.066.431532
AGGGAAT32200.066.11675
ATGAGGG36450.065.9265752
GGTAAGG16000.065.9009551
Produced by FastQC (version 0.11.5)