FastQCFastQC Report
Mon 27 Feb 2023
SRR3128667.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128667.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2600877
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232070.8922759515348093No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG66110.254183492721878RNA PCR Primer, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG58210.22380912284587084RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC51150.19666443280478085RNA PCR Primer, Index 38 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27050.10400338039822721No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69250.083.518091
ATAGCGG5250.074.338531
ATAGGGC15450.073.909013
TAGACGG3900.071.134881
CGAAGGG24150.070.633232
TAGCGGG13650.070.5733642
TATAGGG16550.069.564512
CGTAGGG6700.069.435252
TAGGGCA12000.069.312514
ACGGGTA2650.069.157354
AGTAGGG36500.069.006742
AGGGAAT29050.068.912775
ATAACGG2050.068.811751
GTAGGGA21400.068.7306063
AGACGGG18850.068.555282
AGGGCAT21000.068.4760065
ATAGGGA22650.067.634613
AAGGGTA15550.067.3897864
AGGGATG30200.067.377875
AAGGGAT36250.066.630754
Produced by FastQC (version 0.11.5)