FastQCFastQC Report
Mon 27 Feb 2023
SRR3128666.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128666.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2911068
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291241.0004575640280475No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG71830.24674792893879496RNA PCR Primer, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG64420.22129335350462442RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC56520.19415554703634544RNA PCR Primer, Index 38 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31870.1094787205245635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88300.080.969981
ATAGGGC16300.074.1017463
TAGACGG4500.072.123651
GTAGGGC13150.071.837843
TAGCGGG14750.070.746272
TAGAGGG43800.070.6145862
TATAGGG21200.069.84212
AGTAGGG43650.069.67272
TAGGGCA16600.069.648214
GGTAAGG17250.069.53321
ACGGGTA2650.069.1674354
CGTAGGG7600.068.651812
CGGGTAT3400.067.732965
AAGGGAT45400.067.4954764
CGAAGGG27750.067.2465742
AGGGCAT25350.066.9286655
GTAGGGA23550.066.855453
AGACGGG19900.066.137472
ATAGGGA25300.066.132113
ATAACGG2500.065.852031
Produced by FastQC (version 0.11.5)