FastQCFastQC Report
Mon 27 Feb 2023
SRR3128665.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128665.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1793709
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167350.9329829977995316No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG29780.16602470077364834No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG25400.14160602416556978No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24660.13748049432767523No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC23450.1307346955386855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50300.078.497291
AGTAGGG28450.072.88352
ATAGGGC9500.069.258253
ACGGGTA1050.067.13814
GGATAGG10950.067.045741
GAATAGG11150.066.6872561
AGAAGGG59150.066.6134952
GTAGGGA14700.066.498693
TAGTAGG8800.066.3131561
GGTAAGG9800.066.26951
AGGGTAA7400.066.049385
TAGGGCA9550.065.942974
ATAGCGG4650.065.784121
AAGGGGC49750.065.5591664
AGATAGG9700.065.497191
ATAGGGA16800.065.459643
TAAGGGA12750.065.242433
CGAAGGG18800.064.776112
CGTAGGG5900.064.551442
AGGGCAT15050.064.327675
Produced by FastQC (version 0.11.5)