FastQCFastQC Report
Mon 27 Feb 2023
SRR3128664.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128664.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735617
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154480.8900581176607513No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG33050.1904221956802682No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG28110.16195969502488164No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC26200.1509549629901067No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23250.13395812555419773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48650.080.323161
CGTAGGG4950.073.102592
GAATAGG8750.073.089251
AGTAGGG24450.072.26972
TAGACGG1450.071.347261
ACGGGTA1650.071.203834
GGTAAGG9700.069.809311
TAGGGTA6650.069.255094
AACGAGG7400.068.6301041
ATAGGGC9550.067.9083253
ATAGGGT6450.067.7595443
TAGGGCA9000.067.3588264
AAGGGGC43450.067.165954
ATAGGGA14300.067.0411453
AGATAGG9950.066.637491
TATAGGG13100.066.366312
GGATAGG10450.065.699061
GTAGGGA12300.065.3338553
GTAGGGT6650.065.014983
AGGGTAA8900.064.949365
Produced by FastQC (version 0.11.5)