FastQCFastQC Report
Mon 27 Feb 2023
SRR3128663.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128663.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938322
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188360.971768364595769No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG35050.18082650870185657No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG29740.1534316795661402No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC27150.14006960659787177No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26030.1342914128818638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG1550.084.999331
CGTTTTT55700.080.167841
GAATAGG10500.071.700121
AGGGTAA8300.071.3472445
AGTAGGG30850.071.158192
AGTAAGG12150.070.095711
GGTAAGG12750.069.749451
TAGTAGG9350.069.44751
ACGGGTA1900.069.261074
ATAGGGA17050.068.637223
GCAAGGG25650.068.173992
TATAGGG13400.068.055012
ATAAGGG23650.067.777632
CGTAGGG6400.067.572662
AGGGAAT18650.067.032835
TAGGGCA9750.067.00314
ATAGGGC10500.066.245623
AGAAGGG62950.065.9368442
GAGGGAT20800.065.526864
TAAGGGA14500.065.473893
Produced by FastQC (version 0.11.5)