FastQCFastQC Report
Mon 27 Feb 2023
SRR3128662.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128662.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885699
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289891.0045746281923376No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37710.13067891003185017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90200.079.084911
TAGGGCA12800.073.432174
ATAGGGA26050.072.88533
GGTAAGG15050.072.848461
GAATAGG19150.072.4861
TATAGGG21550.071.5667342
ATAGGGC15800.071.0897753
AGTAGGG42950.070.1745152
CGTAGGG9200.068.9970552
AGTAAGG16000.068.523081
GTAGGGA21500.068.199693
TAAGGGA26600.067.491343
AATAGGG41050.067.466272
AGGGCAT25650.066.8762365
GTAGGGC11350.066.6647643
ATAGCGG6800.066.429851
AGGGATG39900.066.313485
AGGGACT38300.066.2615055
ATAACGG4050.066.2248151
TAGGGAT18200.066.105094
Produced by FastQC (version 0.11.5)