FastQCFastQC Report
Mon 27 Feb 2023
SRR3128661.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128661.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791204
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271790.9737374982265719No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36090.12929904084402286No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG29810.10679978962483574No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG29210.10465017963574143No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC28030.10042261332385595No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86550.080.90281
ATAGCGG5750.074.423391
ATAGGGC15500.072.159453
TAGGGCA12700.072.158354
AGTAGGG43950.070.99982
CGTAGGG8350.070.914012
GAATAGG16800.070.818571
AGGGCAT23000.069.8839345
ATAGGGA24200.068.9383543
TAGAGGG49700.068.837162
GGTAAGG14550.068.518651
TAAGGGA23450.068.337563
AGGGATG39000.068.20735
GTAGGGC10900.067.258323
GTAGGGA20000.067.202273
TATAGGG22350.067.0750662
ATAGAGG19200.066.864761
ATAAGGG33800.066.5988542
AGGGACT33650.066.481525
AGTAAGG13600.066.389321
Produced by FastQC (version 0.11.5)