FastQCFastQC Report
Mon 27 Feb 2023
SRR3128660.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128660.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3157071
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331451.049865524088625No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43100.13651894429995398No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG33680.10668116111420999No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG33120.10490736508618273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100750.080.5705951
TAGGGCA16950.074.309964
ATAGCGG6350.074.1069261
ATAGGGC18250.072.6219943
GAATAGG20400.071.97091
ATAGGGA27700.071.939593
GGTAAGG16800.071.7072751
AGTAGGG50150.070.674852
TAAGGGA28700.070.415533
AGGGATG42500.068.672775
GTAGGGA24950.068.378253
CGTAGGG8750.068.22762
TAGCGGG16100.067.73712
AATAGGG46950.067.5822452
TAGAGGG57300.067.5164642
ATAGAGG21800.067.3489151
ATAAGGG43500.066.350412
CGAAGGG30750.066.03942
ATAACGG3650.065.752131
AGGGCAT27600.065.729475
Produced by FastQC (version 0.11.5)