FastQCFastQC Report
Mon 27 Feb 2023
SRR3128659.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128659.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597329
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337461.2992578144701732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT117100.084.016391
ATAGGGC15400.074.15533
GTACGGG5150.073.022452
ATAGGGA21900.071.244373
AGTAGGG39550.071.195732
GGTAAGG16200.070.2857361
ACGGGAT6050.069.910714
GAATAGG13700.068.687281
TAAGGGA24950.068.374273
TAGGGCA12850.068.0246664
AATAGGG35700.067.02292
TAGAGGG46450.066.9955442
ATGAGGG41200.066.7470862
ATAGCGG5650.066.620581
AGGGATG35550.066.09785
ATAGAGG18150.065.5859451
AGGGAAT26650.065.423425
AGTAAGG17350.065.0846561
GATAGGG37350.064.81722
ATAAGGG35700.063.994362
Produced by FastQC (version 0.11.5)