FastQCFastQC Report
Mon 27 Feb 2023
SRR3128658.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128658.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436730
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308231.2649329224001018No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG26000.1067003730409196No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC24910.10222716509420411No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT24370.10001108042335424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106750.086.2989961
ATAGGGC14650.074.4301453
AGTAGGG39400.070.261532
GAGGGAT30050.070.2263954
GAATAGG16300.069.817931
AATAGGG33750.068.2371442
AGGGATG34000.068.1487055
ACGGGTA1800.067.888994
ATAAGGG31550.067.483462
GTAGGGT7950.067.396333
TAGAGGG45200.067.380642
AGGGATA23000.066.8204655
GGTAAGG14700.066.5403751
AAGGGTA15350.066.136914
TATAGGG18600.065.951712
TAGGGCA11550.065.922184
AGGGAAT25200.065.0900345
ATAGGGA22150.064.505743
AAGAGGG82850.064.4442752
TAGACGG3600.064.007781
Produced by FastQC (version 0.11.5)