FastQCFastQC Report
Mon 27 Feb 2023
SRR3128657.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128657.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724651
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379301.392104897104253No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG29240.10731649668159335No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC27670.10155429080641888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT125300.084.931351
ATAGGGC14950.072.30483
ATAGGGA24650.071.688813
AGTAGGG43700.071.424442
CGTAGGG7250.070.023842
ATAGCGG7300.068.3441851
TAGAGGG47300.067.876552
GTAGGGC12550.067.782153
AGGGAAT28700.067.467685
GAATAGG16000.067.364951
ATAAGGG38200.067.310712
AATAGGG37400.066.613842
AGGGATG37200.066.5806665
TAGGGCA12500.066.1733554
TAAGGGA24700.065.835433
GGTAAGG16900.065.7270051
TGTAGGG19650.065.307082
ATGTAGG8100.065.08061
AGGGTAA11300.064.466445
ATGAGGG42900.063.7713852
Produced by FastQC (version 0.11.5)