FastQCFastQC Report
Mon 27 Feb 2023
SRR3128656.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128656.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679112
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72151.0624168031193677No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCA70431.0370896111392525No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG44040.6484939155838801No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC36270.5340797983248713No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG30480.44882140206622767No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26560.39109896453015114No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATG24450.36002897901966097No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA23690.34883789419123795No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTATGCTCATC16000.23560178586153682No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT12940.1905429443155179No Hit
ACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG12240.18023536618407568No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG9490.13974130923912403No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGC9470.1394468070067971No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG9110.13414576682491255No Hit
AAAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT9040.13311500901176831No Hit
AGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT8950.13178974896629717No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCTATGCTCATCGT8900.13105349338547986No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCATCG7670.11294160609737422No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT7600.11191084828423No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG7580.11161634605190307No Hit
GGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT7000.10307578131442235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT36400.085.920271
TAAGCGG1050.085.230151
ATACCGG500.084.781571
ATAGCGG1450.084.456731
GGTAAGG2800.082.426511
AGTACGG750.081.641511
TAGCGGG3200.080.8531952
AGGGAAT11600.080.218735
CGTAGGG2500.079.030332
CGTACCC301.456081E-678.328064
AGGGCTA3250.078.087045
CTACGAC5900.078.0625465
CGCTACG5900.078.0625463
GGGAATG14950.077.96136
AGGGCAT6150.077.945975
CGAAGGG4700.077.068582
TCGAGGG2950.076.542692
TAGGGCA3200.076.369864
AGAGGGA15100.075.94193
AGGGATT7700.075.683215
Produced by FastQC (version 0.11.5)