FastQCFastQC Report
Mon 27 Feb 2023
SRR3128655.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128655.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658866
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCA91801.3933030388576735No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65010.9866953219622807No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG48740.7397558835939326No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC41340.6274416952764295No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG34780.5278766850922646No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATG32340.4908433581335203No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA27030.4102503392192039No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23710.3598607304064863No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTATGCTCATC19570.29702549532074807No Hit
ACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG14960.22705679151754682No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT14810.224780152565165No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGC11540.17514942340324133No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCTATGCTCATCGT11500.1745423196826062No Hit
AGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT10160.15420434504132857No Hit
AAAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT10040.15238303387942312No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG9390.14251759841910192No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG9020.13690188900322675No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG8200.12445626273020614No Hit
GGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT7970.12096541633655401No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCATCG7880.11959943296512493No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT7870.11944765703496615No Hit
ACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT7600.11534970692067886No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG7450.11307306796829704No Hit
GAACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT7410.11246596424766189No Hit
AGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT7130.10821623820321583No Hit
TAGACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT7000.1062431511111516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG353.5652192E-1094.0600051
ATAGCGG1200.090.140841
TAGCGGG2850.089.041842
CGTAGGG2150.087.431272
CGTTTTT33650.087.0718841
TCCGCTA4150.086.127231
GCGTAGG1550.084.957421
CGGGTAT452.7284841E-1183.545435
AGGGAAT11300.082.344015
GGTAAGG2650.081.636981
CGTACCC353.8653525E-880.5616764
AGGGCTA3000.079.890335
TCGAGGG2600.079.528832
CGCTACG4450.079.203893
TAAGGGA6200.078.071193
TACGACC4550.077.463146
AGGGTAA2550.077.402395
GGGAATG15450.076.650926
AGGGAAC7000.076.533595
TAGAAGG4550.076.488341
Produced by FastQC (version 0.11.5)