FastQCFastQC Report
Mon 27 Feb 2023
SRR3128654.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128654.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715748
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82581.15375802656801No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG56160.7846336978936721No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCA48440.6767745072288012No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC47520.6639208212946456No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG37330.5215522781761178No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28050.39189770701420057No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA15950.2228437941845454No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT15640.21851266088064514No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATG14890.20803411256475743No Hit
ACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG12400.17324533215601023No Hit
AAAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT11970.16723763112156792No Hit
AGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT11700.16346535372784834No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG10300.1439053968715246No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTATGCTCATC10210.1426479710736181No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG9530.13314742060054655No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT9230.12895600127419146No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCATCG8570.11973487875621028No Hit
ACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT8520.11903630886848444No Hit
GGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT8450.11805831102566824No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG8150.11386689169931316No Hit
GAACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT7990.11163146805859045No Hit
TAGACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT7970.11135204010350012No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC7550.10548405304660298No Hit
AGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT7360.10282948747324476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2300.087.877992
CGTTTTT39950.086.575671
CGGGAAT2900.084.272175
GCGTAGG1500.081.5656741
TAGCGGG3500.080.5791552
TCCGCTA4850.080.530721
CTAGCGG950.079.254091
TTCACGG600.078.428521
CGAAGGG4400.077.984752
GGTAAGG2850.077.602971
AGGGAAT13950.077.487645
TACGGGT551.8189894E-1276.905723
GCGGGAT3250.076.642794
CGCTACG5100.076.486853
ACGACCA5050.076.3134847
TAGGGCA4700.075.9966664
GGGAATG18500.075.958836
AGGGAAC8300.075.876195
AGGGCTA3350.075.757875
GCTACGA5150.075.744254
Produced by FastQC (version 0.11.5)