FastQCFastQC Report
Mon 27 Feb 2023
SRR3128653.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128653.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2765901
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258790.9356444789600207No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36380.13153037653914584No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC32390.11710469752894265No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG32120.11612852376133491No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG28580.10332980103047795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87950.080.607431
CGTAGGG7550.075.982132
GAATAGG19700.073.120831
AGGGATG52400.072.91465
TAGGGTA7500.071.432984
GAGGGAT39500.070.4335864
TAGGGCA14250.070.245734
TAAGGGA25050.070.1647643
GTAGGGA22400.070.073473
TACGGGT2550.070.032333
ATAGGGA28050.069.697253
AGGGAAT33400.069.226725
GTAGGGC11750.069.19323
GGTAAGG16600.068.910291
GTACGGG6150.068.812292
AGGGACT34500.067.973025
AAGAGGG94600.067.4508362
AGTAGGG43150.067.3451462
AAGGGAT54850.066.915954
AAGGGTA19550.066.346424
Produced by FastQC (version 0.11.5)