FastQCFastQC Report
Mon 27 Feb 2023
SRR3128652.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128652.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2591983
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240040.9260863207821965No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC36510.1408574053147725No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG36500.14081882481482325No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33840.13055641182831831No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG33190.12804867933161598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81100.081.703551
TAAGGGA25300.074.485713
TAGGGCA13050.074.183244
CGTAGGG7450.073.80382
AGGGATG44800.073.640485
GAATAGG16800.072.500551
GTAGGGC12750.070.399933
GGTAAGG14900.070.383271
GTAGGGA22050.070.119113
GAGGGAT35850.069.869424
CGTACAC12250.069.820734
AGTAGGG41150.068.6362842
ATAGGGA27550.068.0613253
CCGTACA12800.067.922063
AGGGCAT18600.067.7141345
AAGGGAT49250.067.653314
TAGAGGG45800.067.619052
CGAAGGG24500.066.9435042
AGGGAAT34550.066.922875
TAGACGG3800.066.8283841
Produced by FastQC (version 0.11.5)