FastQCFastQC Report
Mon 27 Feb 2023
SRR3128651.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128651.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908635
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293561.0092706716380708No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG41490.14264423002542428No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC39600.13614633668370216No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39180.13470236038554168No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG36810.1265542084173504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99000.081.535091
CGTAGGG9000.075.208632
AGGGATG54200.072.491585
ACGGGTA3050.072.423354
TAAGGGA27100.071.624453
TAGGGTA9050.070.1077044
GAGGGAT39850.070.0549244
GGTAAGG16200.070.019281
AGTAAGG17650.069.600341
GAATAGG19750.069.34921
ATAGGGA31350.069.26043
GTAGGGA25750.069.173943
TAGGGCA16250.069.12344
AGGGAAT34850.069.047465
AGTAGGG46800.069.001512
TAGAGGG50750.068.817762
AGAGGGA69350.068.58273
ATAGGGC18000.067.625083
AAGGGAT54650.066.7347644
AAGGGTA19600.066.420824
Produced by FastQC (version 0.11.5)