FastQCFastQC Report
Mon 27 Feb 2023
SRR3128649.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128649.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996147
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183710.9203230022638613No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG56360.28234393559191784No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50930.2551415301578491No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG48210.24151527918535057No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC44000.22042464808453488No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGCCTGT32270.161661440765635No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA20410.1022469788046672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56500.083.1420751
TAGGGCA7800.076.517834
CGTAGGG6250.073.6884842
ATAGCGG4250.073.0228651
TAAGGGA16250.072.5895163
AGGGCAT16000.071.667695
AGGGAAT25550.070.998625
CTACGAC10600.070.936165
TAAGGGT7950.070.936153
TACGGGT1800.070.4928053
AGTAGGG25350.069.89032
ATAGGGA19700.069.419313
GGTAAGG10300.069.392121
AAGGGCA25500.068.7424
AGGGATG25800.068.671295
TACGACC10950.068.668796
ATAGGGC11750.068.393023
AAGAGGG59600.067.8118742
ATAAGGG25950.067.731052
GTAGGGC8200.067.627243
Produced by FastQC (version 0.11.5)