FastQCFastQC Report
Mon 27 Feb 2023
SRR3128648.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128648.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213297
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230331.0406646735616594No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG60940.27533584512155396No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57270.25875424762243837No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG54260.2451546267852891No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC50030.2260428672699597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67400.083.9240951
AGGGCAT17800.073.400245
CGTAGGG6600.072.645292
GGTAAGG12300.072.310081
TAAGGGA17900.072.202523
AGTAGGG29150.071.7583852
CTACGAC11600.071.7113655
AGGGAAT27850.071.381865
AAGGGCA31050.070.685114
ATAGGGC13000.070.13443
TCCGCTA12300.068.866741
AGGGATG30550.068.765255
AGGGTAC9900.068.359685
ACGACCA12050.068.25337
TAGGGCA10250.067.859484
TAAGGGT7550.067.850383
AAGAGGG66950.067.682692
ATAGCGG4600.067.519351
AGGGTAT12500.067.30015
ATTGCGG2100.067.227051
Produced by FastQC (version 0.11.5)